Coding

Part:BBa_K4623014:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)


Basic Silinker expression pathway（T7）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 520
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

The T7 promoter, RBS and T7 terminator were derived from the pETDuet-1 plasmid skeleton. Other parts were derived from DNA sequences after codon optimization according to the amino acid sequence

References